Application

Research CategoryNeuroscience

Immunoblotting at 1:500 using fresh rat brain extract or enriched microtubule protein preparation.

Immunohistochemistry: 1:50 to 1:1000

Optimal working dilution must be determined by the end user.

Anti-MAP2A Antibody, 2B, 2C, clone HM-2 detects level of MAP2A & has been published & validated for use in WB, IH.

Research Sub CategoryNeuronal & Glial MarkersNeurofilament & Neuron Metabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Rat brain microtubule-associated protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Ascites fluid. Liquid with 15 mM sodium azide.

Specificity

Shows selective staining of dendritic trees throughout the brain in immunohistochemical staining of brain tissue. In perkinje neurons, staining is restricted to the dendrites distal to the initial dendrite segment. No staining of cell body in mature Perkinje cells but strong staining of cell bodies of developing Perkinje cells from neonatal rats. No detectable staining of axons or non-neuronal cells. Reacts specifically with all forms of MAP2 (MAP2a ~280 kDa, MAP2b ~280 kDa and MAP2c ~70 kDa) as determined by immunoblot. No cross-reactivity observed to other MAPs or tubulin.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.